Evaluation of Cytotoxicity of ProRoot MTA and Endocem at Different Times and Concentrations on Human Gingival Fibroblasts

Background and Aim: The aim of this study was to compare the cytotoxicity of ProRoot MTA and Endocem at different times and concentrations on human gingival fibroblasts. Materials and Methods: In this in-vitro study, ProRoot MTA and Endocem extracts with concentrations of 3, 6, 12, 25, and 50 mg/ml in the unhardened state (solution) at 24, 48, and 72 hours on human gingival fibroblasts were transferred to 96-well plates in Dulbeccochr('39')s Modified Eaglechr('39')s medium (DMEM). For each material at any time and any concentration, three wells were used, and 90 samples were examined. Also, to calibrate the cytotoxicity measuring device at 24, 48, and 72 hours, 3 wells for positive control and 3 wells for negative control have been considered, which include a total of 9 positive control wells and 9 negative control wells. Samples were analyzed by MTT assay. Data were analyzed by analysis of variance (ANOVA). Results: In the present study, the samples in the two groups at different times and concentrations did not show a significant difference in terms of cytotoxicity (P<0.05). The highest cytotoxicity was related to Endocem at 24 hours and a concentration of 50 mg/ml, and the lowest was related to ProRoot MTA at 72 hours and a concentration of 6 mg/ml. Conclusion: According to the research, in general, the degree of cytotoxicity of Endocem is comparable to that of ProRoot MTA.